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glutathione and crohn's disease Frontiers stage: IVF
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Larger and better diffracting pyramid-shaped crystals were obtained at 8 °C after 3 to 4 weeks in a crystallization buffer containing 25 mM Tris pH 8.5 and 25 to 30% (v/v) PEG400, 50 mM Tris pH 8.5 and 27 to 30% (v/v) PEG400 or 75 mM Tris pH 8.5 and 29 to 30% (v/v) PEG400, using the sitting drop vapour diffusion method (in MRC Maxi 48-well plate) and a 1:1 mixing ratio (2 μl final drop volume) of mother liquor and protein solution
stage: IVF
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