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It was found that the 4' − OH on the B ring of the four hydroxyl groups had the lowest bond dissociation enthalpy (BDE) value.4' − OH was the strongest antioxidant hydroxyl group in luteolin, which was more involved in the free radical scavenging process of luteolin than other OH groups
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Virus was purified on a sucrose gradient, pelleted, and resuspended in 10% sucrose in D-PBS
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