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[00102] In general, techniques identifiable by skilled persons that can be used to perform fractionating proteins or digested proteins of a biological sample comprise methods based on purification of peptides according to their isoelectric points (e.g., by running them through a pH graded gel or an ion exchange column), separation according to their size or molecular weight (e.g., via size exclusion chromatography or by SDS-PAGE (sodium dodecyl sulfate- polyacrylamide gel electrophoresis) analysis), or separation by polarity/hydrophobicity (e.g., via high performance liquid chromatography or reversed-phase chromatography)
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