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Cell lines and culture All cells were cultured in DME/F12 (50:50) mixed medium supplemented with 10% fetal calf serum, 2 mM glutamine, 1 mM pyruvate, 100 U ml −1 penicillin and 100 μg ml −1 streptomycin at 37 °C in a humidified incubator under 5% CO 2

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Other natural extracts such as grape seed extract, green tea extract, and rosehip extract are incorporated to nourish the skin and protect it from environmental stressors