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After incubation with cell lysate, the resin was washed with PBS, and the bound protein was eluted with the elution buffer (50 mM Tris-HCl, pH 8.8, 20 mM reduced glutathione, 300 mM NaCl, and 0.1% Triton X-100)
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In addition to the widely-used biotin-streptavidin pair, frequently utilized are recognition pairs based on protein affinity purification handles, such as hexahistidine, FLAG, and GST tails, as well as specific antibodies targeting post-translational modifications, such as various histone methylation loci
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