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(2) molecular dynamics simulated annealing restraining FOXO4 FH (without flexible regions) harmonically to its template structure (using a non-crystallographic force constant of 10,000 kcal mol β1 Γ β2 ), with additional dihedral angle restraints from 1 H, 15 N, and 13 C chemical shifts using TALOS-N 56 , hydrogen bond restraints (region p53 residues 48-55
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Key Takeaways Semax is a synthetic fragment of the ACTH hormone studied mainly for stroke recovery and attention, with mechanistic links to semax bdnf signaling
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