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LDH was measured in both the culture medium and cell lysates, and LDH release (%) is expressed as (LDH levels in culture medium)/([LDH levels in culture medium] + [LDH levels in cell lysis]) × 100
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Precipitated proteins were washed with cold acetone and air dried before reconstitution into 40 µL of 8 M urea in 50 mM Tris-HCl (pH 8)
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