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Not all B12 is the same. Cyanocobalamin needs conversion. Some people can't do it efficiently. Methylcobalamin is ready to use — especially for brain and nerves. Adenosylcobalamin is for cellular energy production.
Whereas immunohistochemical localization using fluorescence probe as secondary antibody can provide localization a relative abundance with high sensitivity, LC-ES/MS is the technique that provides a most consistent quantification
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