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A cocktail of 11 sarbecovirus antigens and 1 control proteins (HIV-1 antigens), obtained commercially (SinoBiological) or internally produced (see below), spanning Spike S1 and S2 domains for SARS-CoV-2 or SARS-CoV-1 were covalently coupled to uniquely coded magnetic microspheres (Luminex) per manufacturer’s protocol and added to the plate in a final volume of 50 μl/well
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The blend combines CJC-1295 without DAC and Ipamorelin in one vial at a fixed ratio, while the stack supplies each in its own vial
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