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The “lytic” mechanism of NET formation is the combination of a ligand with the cellular receptor, which initiates Raf-MEK-ERK signal transduction and mobilizes the NADPH oxidase complex
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After incubation with Ni-NTA Superflow beads (Qiagen) for removal of the cleaved tags and the TEV protease, the supernatant was concentrated to 1.5 mL, frozen in liquid nitrogen, and stored overnight
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